產(chǎn)品概述

單核細(xì)胞趨化蛋白1(MCP-1/CCL2)，也稱為單核細(xì)胞趨化與激活因子（MCAF），是趨化因子家族CC亞族一個(gè)成員。MCP-1是一種很有潛能的單核細(xì)胞及巨噬細(xì)胞的誘生劑及激活因子。人MCP-1 cDNA編碼99個(gè)氨基酸組成的前體蛋白，當(dāng)剪切掉23個(gè)氨基酸疏水性信號(hào)肽后，就形成了由76個(gè)氨基酸組成的成熟蛋白。與小鼠趨化因子相比，人MCP-1與小鼠MCP-5（CCL12）、MCP-1/JE(CCL2)最相關(guān)，小鼠MCP-5與人MCP-1有65%序列同源性。MCP-1可以被許多炎癥介導(dǎo)物誘導(dǎo)，如LPS、 IL-1β、 TNF-α、IFN-γ、IL-6及IL-4等。表達(dá)MCP-1的細(xì)胞有：單核細(xì)胞、肥大細(xì)胞、T細(xì)胞、成骨細(xì)胞、成纖維細(xì)胞、內(nèi)皮細(xì)胞、表皮細(xì)胞、小膠質(zhì)細(xì)胞、星形膠質(zhì)細(xì)胞及平滑肌細(xì)胞等。

產(chǎn)品原理

本實(shí)驗(yàn)采用雙抗體夾心ELISA法。抗人MCP-1單抗包被于酶標(biāo)板上，標(biāo)本和標(biāo)準(zhǔn)品中的MCP-1會(huì)與單抗結(jié)合形成免疫復(fù)合物，游離的成分被洗去；加入生物素化的抗人MCP-1抗體，生物素化抗人MCP-1抗體與酶標(biāo)板上結(jié)合的標(biāo)準(zhǔn)品或樣本中的MCP-1結(jié)合而形成免疫復(fù)合物，游離的成分被洗去；加入辣根過氧化物酶標(biāo)記的親合素，親合素與生物素特異性結(jié)合，游離的成分被洗去。加入顯色底物（顯色劑），若反應(yīng)孔中有MCP-1，辣根過氧化物酶會(huì)使無色的顯色劑變藍(lán)，加終止液變黃。在450nm處測OD值， MCP-1濃度與OD₄₅₀值之間呈正相關(guān)，可通過繪制標(biāo)準(zhǔn)曲線求出標(biāo)本中MCP-1濃度。

產(chǎn)品性能

1. 靈敏度：最小可測人MCP-1達(dá)7pg/ml 。

2. 重復(fù)性：板內(nèi)、板間變異系數(shù)均<10%。

產(chǎn)品組分及儲(chǔ)存條件

試驗(yàn)所需自備儀器和試劑:

1. 酶標(biāo)儀(450nm檢測波長濾光片，570nm或630nm校正波長濾光片)。

2. 高精度加液器及一次性吸頭：0.5-10μl，2-20μl，20-200μl，200-1000μl。

3. 37℃恒溫箱、蒸餾水或去離子水、坐標(biāo)紙。

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